Software:ProbID

Overview

ProbID is a software tool designed to identify peptides from tandem mass spectra using a protein sequence database. It computes z scores for the identifications and output the results in pepXML format, which subsequently can be analyzed by the TPP (Trans-Proteomic Pipeline). ProbID is written in Java.

Getting the software

Downloading the package

You can download from the Sashimi File Release page on SourceForge. The package includes

• ProbIDALL.jar This is the main package of software to identify peptides from MS/MS spectra using protein sequence database (FASTA format). This jar file includes both the source code and binary class files.
• probid.param This is the parameter file for doing peptide searches.
• example.dta This is the file format of MS/MS spectra that ProbID takes.
• runprobid.pl This is the script that takes a your_file.mzXML and returns a your_file.xml which can be analyzed by TPP (Trans-Proteomic Pipeline).
• runprobiddta.pl This is a script that batch processes all your .dta files in a directory.
• README basic information.

Source Code

Source is accessible from Sashimi SVN (add svn link).

Installation

Assuming you've downloaded the package, first unzip ProbID.tgz:

tar -xzf ProbID.tgz

• set up "CLASSPATH" environment variable for ProbIDALL.jar
• you need either jre1.4 (or above) or j2sdk1.4 (or above) installed on your machine.
• ProbID was written in java and can be run on almost any OS.

Running ProbID

ProbID may be run in single-spectrum or batch processing modes. Both options require you to have probid.param in the same directory where your data is located.

single MS/MS spectrum processing

java org.systemsbiology.dbsearch.ProbID your_file.dta

OR if you didn't set up the "CLASSPATH" variable

Java -cp where_ProbIDALL.jar_is org.systemsbiology.dbsearch.ProbID your_file.dta

batch processing of many .dta files on one computer

perl runprobid.pl your_file.mzXML. 

You do need "mzxml2other" installed on your machine to start from your_file.mzXML. If you already have all .dta files in current directory, use runprobiddta.pl.

perl runprobiddta.pl

Note: should we recommend MzXML2Search instead of mzxml2other?

ProbID and the TPP

Please see the ProbID and the TPP page.

Reference

Coming soon...