TPP 5.1.0: Installing on Ubuntu 16.04 LTS
From SPCTools
 | Note that this recipe will likely not be exactly applicable to different versions of TPP or different distributions of Linux, but perhaps can be a useful head start. For other versions of TPP, please see the README and INSTALL_LINUX files found in source code distribution for instructions on how to build TPP for the Linux platform. |
Contents |
Before we begin
This recipe describes installing TPP release 5.1.0.
First some general notes
- We assume a fresh installation of Ubuntu 16.04 LTS as a starting point (LTS stands for Long Term Support, which means that this version of Ubuntu will be stable and supported for several years).
- This recipe was tested on a fresh Azure cloud computing platform instance running Ubuntu 16.04 LTS in December 2017
- We assume that there is an ordinary user account named 'tpp' that will be used as the primary account. This account will need sudo privileges.
- This installation assumes you are installing to
/local/tpp/, if you want to install anywhere else, change accordingly - This installation assumes you are using Apache 2.4 as your web server
- The gray blocks are commands to be typed in at the shell directly. You should be able to copy and paste them a line at a time.
Installation
Installing prerequisite packages
Use the ubuntu package manager to upgrade any outdated packages and then install all the dependencies
sudo apt update sudo apt --yes upgrade sudo apt --yes install subversion sudo apt --yes install make sudo apt --yes install g++ sudo apt --yes install build-essential sudo apt --yes install zlib1g-dev sudo apt --yes install libghc-bzlib-dev sudo apt --yes install gnuplot sudo apt --yes install unzip sudo apt --yes install expat sudo apt --yes install libexpat1-dev
Creating a suitable place to compile and install
In this recipe, we install in /local. You may wish to install in a different location, and the recipe should be adjusted accordingly. This also assumes that you will use an account 'tpp' and group 'tpp'. Maybe you'll use a different account.
sudo mkdir /local cd /local sudo mkdir tpp data svn sudo chown tpp.tpp tpp data svn
Pulling the TPP 5.1.0 source code from SourceForge
cd /local/svn svn checkout svn://svn.code.sf.net/p/sashimi/code/tags/release_5-1-0
Compiling the source code
Create a custom site.mk file with something like the following. After pasting in the three data lines, hit CTRL-D.
cd /local/svn/release_5-1-0 cat > site.mk INSTALL_DIR = /local/tpp TPP_BASEURL = /tpp TPP_DATADIR = /local/data
First make libgd.
make libgd
Then make everything else. This will start by unpacking Boost and ProteoWizard and compile all that and then compile the TPP. This takes quite a while.
make all
If the above completed without error, then you're ready to proceed. If the compilation stopped with an error, you will need to troubleshoot. Perhaps some prerequisite is not installed on your system. It has been reported that sometimes just doing another `make all` will allow completion of compilation. Try that first before trying something more complex. Next, install it!
make install
Next you'll need to make sure that all the Perl modules are available. The following works on the fresh version of Ubuntu 16.04 LTS that we tried on:
sudo cpan
(answer yes)
make install
install Bundle::CPAN
(this takes a long time. At one point you need to hit [ENTER] to accept the 'exit')
install CGI
install XML::Parser
install FindBin::libs
install JSON
(you may need to answer 'y' to petulant question)
quit
Now test to make sure all modules are installed:
cd /local/tpp/cgi-bin ./tpp_gui.pl
If it compiles and you get an error about being unable to find HomePath then you're fine.
However, if you get an error not being able to find a module, then go back to cpan and install it.If you have no interest in using the web interfaces, then you're done. Happy TPPing. However, if you want the web interfaces, install and configure Apache.
Configuring the Apache web server
The following assumes you will install on port 10401. Substitute 10401 for 80 if you want to install on the default port.
sudo apt --yes install apache2 cd /local/tpp/conf cat httpd-tpp.conf | sed 's/_TPP_PORT_/10401/' > httpd-tpp-1.conf cat httpd-tpp-1.conf | sed 's/ScriptInterpreterSource/#ScriptInterpreterSource/' > httpd-tpp-2.conf vi httpd-tpp-2.conf Comment out: # PassEnv TPP_HOME # PassEnv TPP_DATADIR UNcomment these lines (and make sure they have leading slashes!): SetEnv TPP_HOME /local/tpp SetEnv TPP_DATADIR /local/data SetEnv TPP_BASEURL /tpp SetEnv TPP_DATAURL /tpp/data
Next configure the permission access to the server. If only the localhost should be able to access the interface (e.g. installation on a laptop running Linux then only grant access to 127.0.0.1. Or you can just give access to a range of IP addresses. Or you could grant world access if you have collaborators everywhere accessing the system. This confers some additional possible security risks. In this example, we've used the Apache 2.4 syntax to grant access to localhost (127.0.0.1) and IP addresses 174.*.*.*. Access to all has been commented out:
<Directory "/local/tpp">
AllowOverride None
Require ip 127.0.0.1
Require ip 174
# Require all granted
</Directory>
Also do the same for the data directory:
<Directory "/local/data">
Options Includes Indexes MultiViews
Require ip 127.0.0.1
Require ip 174
# Require all granted
AddType text/html .shtml
AddHandler server-parsed .shtml
</Directory>
Next set up some needed Apache modules and restart:
sudo a2enmod rewrite sudo a2enmod cgid sudo service apache2 restart
Next install this config file. This may vary among distributions, but this seems like a nice way to do it on Ubuntu 16.04.
cd /etc/apache2/conf-available sudo cp -p /local/tpp/conf/httpd-tpp-2.conf httpd-tpp.conf cd ../conf-enabled sudo ln -s ../conf-available/httpd-tpp.conf sudo service apache2 reload
If that reload generated an error, you'll need to troubleshoot your configuration. Next, set some appropriate permissions for the web server to write. With the first command, verify that your Apache server is running as the user www-data. If it's running as a different user, then substitute that.
ps aux | grep apache set tmpUser=www-data cd /local/tpp sudo chown -R $tmpUser.$tmpUser data log users
And now you're good to go ... head on over to http://localhost:10401/tpp/cgi-bin/tpp_gui.pl or if this is installed on a different machine, use its name or IP address, e.g. http://40.83.177.49:10401/tpp/cgi-bin/tpp_gui.pl. Note that the above installation assumes installation on port 10401. If you chose a different port, then adjust the above URLs accordingly.
Testing the installation with a sample dataset (optional)
If you want to test your installation with a search and a PeptideProphet processing, try this:
Navigate to: http://xxx.xxx.xxx.xxx:10401/tpp/cgi-bin/tpp_gui.pl - Login with guest and password [Files] [Create new directory] tests Go into tests ## On the command-line: ssh tpp@xxx.xxx.xxx.xxx cd /local/data/tests sudo chmod a+w . wget http://www.peptideatlas.org/export/refData/QuickYeastUPS1.zip unzip QuickYeastUPS1.zip sudo chown -R www-data.www-data . Back in web interface [Files] - Go to /local/data/params/ - Check box next to comet.params - Click (Copy) button - Go to /local/data/tests/QuickYeastUPS1 - Check box next to comet.params - Click (Delete) button - Confirm by clicking (Confirm Delete) at top - Click (Paste) button - On the same line as comet.params, click [Params] Make the following changes: peptide_mass_tolerance = 20 peptide_mass_units = 2 isotope_error = 1 - (Save Search Parameters File) [Pipeline: Comet] [TPP Tools] [Comet Search] Choose mz[X]ML Input Files [Add Files] Select /local/data/tests/QuickYeastUPS1/UPS1_50000amol_R1.mzML Choose Comet Parameters File [Add Files] Select comet.params Choose a sequence database [Add Files] Select Yeast_UPS_cRAP.fasta [Run Comet Search] [TPP Tools] [Analyze Peptides] - (Add Files) - Check box next to UPS1_50000amol_R1.pep.xml - (Select) - Check box next to "Use accurate mass binning" - Scroll to bottom and (Run XInteract) - [Refresh] - Make sure no errors - click on [PepXML] link in /local/data/tests/QuickYeastUPS1/interact.pep.xml [ PepXML ] - Click on a probability - Click on a value in the Ions column
