Software:Libra

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Contents

Getting the software

This software is included in the current TPP distribution.

In a nutshell

Libra is a module within the trans-proteomic pipeline to perform quantification on MS/MS spectra that have multi-reagent labeled peptides. More specifically, at ISB we use Libra on MS/MS spectra of iTRAQ labeled samples.

More detail

More detailed information can be found at Sourceforge, including:

  • Command line syntax for using Libra
  • The condition file
  • Details about what Libra does
  • How accurate is the quantitation?

Generating a Condition File

You can generate a condition file using the built-in utility within the TPP web UI (Petunia), or by using a text editor.

Authors

  • Patrick Pedrioli: original code author of Quantitation
  • Andrew Keller: peptide assignment to proteins within pipeline
  • Nichole King: code maintenance/additions/corrections
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