Software:Libra
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| Revision as of 00:11, 10 February 2012 Luis (Talk | contribs) (→In a nutshell) ← Previous diff |
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| ==More detail== | ==More detail== | ||
| - | More detailed information can be found at [http://sashimi.svn.sourceforge.net/viewvc/*checkout*/sashimi/trunk/trans_proteomic_pipeline/src/Quantitation/Libra/docs/libra_info.html Sourceforge], including: | + | More detailed information can be found by [http://sourceforge.net/p/sashimi/code/HEAD/tree/trunk/trans_proteomic_pipeline/src/Quantitation/Libra/docs/libra_info.html downloading this file], including: |
| * Command line syntax for using Libra | * Command line syntax for using Libra | ||
Current revision
Contents |
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Getting the software
This software is included in the current TPP distribution.
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In a nutshell
Libra is a module within the trans-proteomic pipeline to perform quantification on MS/MS spectra that have isobaric multi-reagent labeled peptides. More specifically, at ISB we use Libra on MS/MS spectra of iTRAQ labeled samples; it can also be used on TMT labeled samples. There is no limitation to the number of channels that Libra can quantitate against.
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More detail
More detailed information can be found by downloading this file, including:
- Command line syntax for using Libra
- The condition file
- Details about what Libra does
- How accurate is the quantitation?
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Generating a Condition File
You can generate a condition file using the built-in utility within the TPP web UI (Petunia), or by using a text editor.
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Authors
- Patrick Pedrioli: original code author of Quantitation
- Andrew Keller: peptide assignment to proteins within pipeline
- Nichole King: code maintenance/additions/corrections
