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  1. Protein identification terminology (4723 bytes)
    1: ...to be highly redundant. Thus, we label each Atlas protein using the terminology below.
    5: ...er 2009. This implementation allows you to select proteins based on the terminology below.
    7: ===Protein Presence Levels===
    8: Taken together, the set of proteins with a Presence Level label for any Atlas has th...
    17: ... selection process, each set of indistinguishable proteins is considered to be a single entity, and the one...

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  1. Main Page (6445 bytes)
    25: * statistical validation of peptide and protein identifications;
    33: ==The [[Software:Firegoose, Gaggle, and PIPE|Protein Information and Property Explorer (PIPE)]]==
    36: Once protein identifiers have been established with the TPP, t...
    67: engines and protein sequences. All results of sequence and spectral l...
  2. TPP:V5Feedback (11460 bytes)
    42: * <s>legacy proteinprophet results: prot_wt_xml.pl: "The system canno...
    44: ** pvalue link to interact-pval.png is broken. (look in PValueModel.cxx)
    80: * <s>Lumping these all together under "protein identifiers don't seem to be parsed correctly. C...
    81: ** <s>Clicking on the protein link in the pepxmlviewer when viewing spectrast r...
    84: ** Clicking on the protein link in the pepxmlviewer when viewing peptideprop...
  3. Software:Prequips (1386 bytes)
    1: [[Image:Prequips_icon.png|thumb|Prequips logo.]]
    5: ...ss spectrometry data at the raw data, peptide and protein level. Furthermore, this data can be linked to ot...
  4. Software:Prequips:Inclusion List Builder (3897 bytes)
    10: [[Image:Ilb_concept.png|alt Inclusion List Builder concept]]
    32: ...clusion list runs, the corresponding peptide (and protein) identification in BioWorks tabular file format a...
    57: image:Ilb_basics_01.png|Master table, filters and feature details.
    58: image:Ilb_basics_03.png|Segment information, inclusion list overview and ...
    59: image:Ilb_basics_02.png|Master table and feature details with mapping inf...
  5. TPP:Developer Documentation (23931 bytes)
    52: ...e XSLT processor, Xalan, will also work fine with ProteinProphet. If you use it, just make sure it is inst...
    69: *libpng www.libpng.org
    234: #Go into database to retrieve all proteins corresponding to identified peptides
    236: #ProteinProphet
    237: ProteinProphet.pl interact.xml interact-prot.shtml XML_IN...
  6. Software:Overview (3074 bytes)
    9: ...ophet|PeptideProphet]], [[Software:ProteinProphet|ProteinProphet]], [[Software:XPRESS|XPRESS]], [[Software:...
    44: ==Protein Crosslinking==
  7. TPP:Files and Formats (299 bytes)
    5: ;protXML: interchange of protein-level data; ISB-developed
  8. TPP:7.0.0 Release Notes (6884 bytes)
    21: ...roblem with not shuffling and outputting the last protein in the database and outputting the penultimate tw...
    49: === [ProteinProphet:] ===
    50: ...umber of top peptide probabilities to compute the protein probability, 0 retains old behavior and is still ...
    52: ...X calculation of protein length and coverage when protein accession ends in a bar character
    53: * BUGFIX * parsing protein descriptions from fasta files running on CentOS (...
  9. Software:ProteinProphet (6941 bytes)
    5: ...riginally developed at the SPC, part of the ISB. ProteinProphet is an integral part of the Trans-Proteomic...
    8: ...when one peptide corresponds to several different proteins.
    13: NOPLOT: do not generate plot png file
    14: NOOCCAM: non-conservative maximum protein list
    18: ... GROUPWTS: check peptide's total weight in the Protein Group against the threshold (default: check pepti...
  10. Software:XPRESS (1756 bytes)
    5: ... for quantifying ratios of differentially labeled proteins.
    8: ...XPRESS ratio present in a dataset and correct the protein expression averages by these average and median r...
    11: ...e profiling of differentiation-induced microsomal proteins using isotope- coded affinity tags and mass spec...
  11. Software:ASAPRatio (1791 bytes)
    6: Automated Statistical Analysis on Protein Ratio (ASAPRatio)
    9: ... software quickly generates a list of interesting proteins based on their relative abundance, which is the ...
    12: ...sold R. (2003) "Automated statistical analysis of protein abundance ratios from data generated by stable is...
  12. Software:TPP (8915 bytes)
    14: ....proteomecenter.org/wiki/index.php?title=Software:ProteinProphet]]
    32: |[[Image:Info.png|middle|left]]
    63: [[Software:ProteinProphet|ProteinProphet]]: Protein identification and validation, using [[Software:P...
    67: ===Protein Quantification===
    69: ...alculation of relative abundances of peptides and proteins from isotopically labeled MS/MS samples.
  13. Software:XLink (3210 bytes)
    4: ...data derived from digested samples of crosslinked protein complexes.
    14: ...database of crosslinker-modified species based on protein sequence(s), crosslinker and protease(s). Once cl...
    24: "Integrative Protein Crosslinking Analysis Using Mass Spectrometry, Is...
  14. Software:PeptideSieve (6357 bytes)
    8: ...accurately identify proteotypic peptides from any protein sequence and offer starting points for generating...
    10: ...e sequences. The program then returns which of a protein's peptides are most likely to be proteotypic for ...
    13: ...ram first performs an ''in silico'' digest of the protein and then converts each of the peptides into chemi...
    64: ...Observed_Proteins.txt AEBERSOLD_TABLE_S1_Observed_Proteins]
  15. Formats:protXML (124 bytes)
    1: A format for storage, exchange, and processing of protein identifications created from ms/ms-derrived pepti...
  16. Software:Out2XML (2805 bytes)
    35: ...b.fasta if running TPP on Linux. Thus, viewing of protein sequences in the TPP is impossible.
  17. Software:Mascot2XML (2806 bytes)
    24: -desc : generate protein description in pepXML output
    25: ...rotein id as per Mascot result (default uses full protein ids in fasta file)
    51: protein_endopeptidase : cut(P) nocuts(-) sense(C)
  18. TPP:X!Tandem and the TPP (5454 bytes)
    49: GROUP, protein
  19. Software:SpectraST (70083 bytes)
    7: ...orkflows, commonly known as shotgun proteomics, a protein sample of interest is first digested with a prote...
    9: ...abase) searching. In sequence searching, a target protein (or translated DNA) database is used as a referen...
    17: ...lational modification sites, if specified -- in a protein database are considered. Most of these putative p...
    53: ...ing peptide identifications of library entries to protein sequence database of choice
    85: ...ortation, automatic validation of search results, protein inference, and quantification and visualization.
  20. Spectrast create.params (4398 bytes)
    25: # Use protein list to filter library
    26: useProteinList =
    38: # Refresh protein mappings against FASTA file specified

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