Msconvert Capabilities

From SPCTools

Revision as of 23:27, 19 May 2010; view current revision
←Older revision | Newer revision→
Jump to: navigation, search

Testing of MSconvert is in progress and results will be posted here as they come.


Contents

Agilent

Raw file type: .d directory
raw-to-mzXML converter: trapper

trapper

mzXML

Conversion: OK
X!Tandem search: Tandem Search completed
TPP:

msconvert

mzXML

Conversion: Conversion completed
X!Tandem search:Tandem Search completed
TPP:

mzML

Conversion: Conversion Completed
X!Tandem search: Tandem Search completed
TPP:



Thermo

Raw file type: .RAW file
raw-to-mzXML converter: ReAdW

ReAdW

mzXML

Conversion: using remoteconvert.pl --convertWith readw, file successfully converted.
X!Tandem search:search ran successfully, slight differences with the msconvert mzXML file
TPP:TPP Search successful--calctppstat for mzXML by ReAdW similar to that of calctppstat for mzXML file by msconvert, slight differences, histograms are relatively different, numbers are relatively close to one another, through most numbers are different--a few number are the same, results are very close--see below(section msconvert: mzXML)

msconvert

mzXML

Conversion: Appears to be successful
X!Tandem search: Search completed, slight differences with the ReAdW converted mzXML file
TPP:Peptide prophet completed with warnings,protein prophet failed. Search completed----calctppstat for mzXML by ReAdW similar to that of calctppstat for mzXML file by msconvert, slight differences, histograms are relatively different, numbers are relatively close to one another, through most numbers are different--a few number are the same, results are very close, comparisons are below:

Calctppstat results for ReAdW file conversion

 138 regis /proteomics/gsun/raw-test/ReAdW> /regis/sbeams/bin/calctppstat.pl --write --full --FDRthresh interact-prob.pep.xml
 INFO: Reading ProteinProphet file 'interact-prob.prot.xml'
 PepP      25/  2621  0.010   | ProP       9 (    16,    25)    /proteomics/gsun/raw-test/ReAdW
 P threshold for FDR 0.01=0.98
 Decoy results:
 PP Incor FDR 0.01=0
 Decoy IDs at FDR 0.01=0
 Decoy IDs at P<0.001=-1
 Total IDs at P<0.001=1493
 Decoy fraction P<0.001=-0.0007
 At PepPro FDR 0.010 FDR based on decoys=-0.0000
 FDR after discard=-0.0000
 For FDR 0.01, sensitivity=0.160 at P=0.98
 Peptide Count at FDR 0.01:
 Distinct peptides = 16
 Singletons = 12 (0.75)
 Doubletons = 2 (0.12)
 Number of PSMs per MSrun:
 hui052703_15_1                          :     25
 Charge 1: 8  (FDR 0.01)       Queries=146
 Charge 2: 11  (FDR 0.01)      Queries=2475
 Charge 3: 6  (FDR 0.01)       Queries=2475
 Enzymatic Termini 1: 17
 Enzymatic Termini 2: 8
 Fraction semi-tryptics: 0.6800
 N missed cleavages 0: 22  (FDR 0.01)
 N missed cleavages 1: 1  (FDR 0.01)
 N missed cleavages 2: 2  (FDR 0.01)
 Fraction of missed cleavages: 0.1200
 Total Modifications at FDR 0.01:
 Modification: C[160]: 5
 Modification: M[147]: 8
 Modification: None: 14
 Mass diffs for P > 0.98: Mean: 1.349  StDev: 0.981512735526137
 Mass diffs for P > 0.98: Min: -0.154  Max: 3.230
 -0.750 -> -0.250      0 |
 -0.250 ->  0.250      5 |*****************************************
  0.250 ->  0.750      1 |********
  0.750 ->  1.250      6 |**************************************************
  1.250 ->  1.750      3 |*************************
  1.750 ->  2.250      5 |*****************************************
  2.250 ->  2.750      3 |*************************
  2.750 ->  3.250      2 |****************
  3.250 ->  3.750      0 |
 ProteinProphet info:
 Proteins identified at P>0.9=9
 Distinct modified peptides at P>0.98=16
 P threshold for protein group FDR 0.01=0.9541
 Protein groups identified at FDR 0.01=8
 Decoy IDs P>0 at protein group FDR 0.01=0 (0.0000)
 Decoy IDs P>0 at protein group FDR 0.03=0 (0.0000)
 Decoy IDs P>0 at protein group FDR 0.04=0 (0.0000)

Calctppstat results for msconvert file conversion

 143 regis /proteomics/gsun/raw-test/msconvert> /regis/sbeams/bin/calctppstat.pl --write --full --FDRthresh interact-prob.pep.xml
 INFO: Reading ProteinProphet file 'interact-prob.prot.xml'
 PepP      18/  2621  0.007   | ProP      10 (    13,    23)    /proteomics/gsun/raw-test/msconvert
 P threshold for FDR 0.01=0.98
 Decoy results:
 PP Incor FDR 0.01=0
 Decoy IDs at FDR 0.01=0
 Decoy IDs at P<0.001=-1
 Total IDs at P<0.001=1481
 Decoy fraction P<0.001=-0.0007
 At PepPro FDR 0.010 FDR based on decoys=-0.0000
 FDR after discard=-0.0000
 For FDR 0.01, sensitivity=0.110 at P=0.98
 Peptide Count at FDR 0.01:
 Distinct peptides = 10
 Singletons = 6 (0.60)
 Doubletons = 2 (0.20)
 Number of PSMs per MSrun:
 hui052703_15_1                          :     18
 Charge 1: 8  (FDR 0.01)       Queries=146
 Charge 2: 4  (FDR 0.01)       Queries=2475
 Charge 3: 6  (FDR 0.01)       Queries=2475
 Enzymatic Termini 1: 12
 Enzymatic Termini 2: 6
 Fraction semi-tryptics: 0.6667
 N missed cleavages 0: 15  (FDR 0.01)
 N missed cleavages 1: 1  (FDR 0.01)
 N missed cleavages 2: 2  (FDR 0.01)
 Fraction of missed cleavages: 0.1667
 Total Modifications at FDR 0.01:
 Modification: C[160]: 4
 Modification: M[147]: 6
 Modification: None: 10
 Mass diffs for P > 0.98: Mean: 1.373  StDev: 1.09399618237854
 Mass diffs for P > 0.98: Min: -0.154  Max: 3.230
 -0.750 -> -0.250      0 |
 -0.250 ->  0.250      5 |**************************************************
  0.250 ->  0.750      0 |
  0.750 ->  1.250      2 |********************
  1.250 ->  1.750      3 |******************************
  1.750 ->  2.250      4 |****************************************
  2.250 ->  2.750      2 |********************
  2.750 ->  3.250      2 |********************
  3.250 ->  3.750      0 |
 ProteinProphet info:
 Proteins identified at P>0.9=10
 Distinct modified peptides at P>0.98=13
 P threshold for protein group FDR 0.01=0.9484
 Protein groups identified at FDR 0.01=9
 Decoy IDs P>0 at protein group FDR 0.01=0 (0.0000)
 Decoy IDs P>0 at protein group FDR 0.02=0 (0.0000)
 Decoy IDs P>0 at protein group FDR 0.03=0 (0.0000)

mzML

Conversion: Conversion seemed to work. TPP tests did not work with mzML file format
X!Tandem search:
TPP:TPP Search failed



Analyst

Raw file type: .wiff file
raw-to-mzXML converter: mzWiff

mzWiff

mzXML

Conversion: used /regis/sbeams/bin/remoteconvert.pl --default --convertWith mzWiff QS20061230_P0094_C1_08_AQv1.1.wiff, file converted, but not output files created or received. Zip file empty. Tried using ep server, new problem arose. Zhi says that mzWiff has many problems and some files it just cannot convert. This is probably what is happening.
X!Tandem search:
TPP:

msconvert

mzXML

Conversion:Tried /regis/sbeams/bin/remoteconvert.pl --default --convertWith msconvert QS20061230_P0094_C1_08_AQv1.1.wiff, could not connect with server. Also tried /regis/sbeams/bin/remoteconvert.pl --default --server th --convertWith msconvert QS20061230_P0094_C1_08_AQv1.1.wiff, also could not connect to server. Tried using both actual file and symbolic link for .wiff file; same problem as above.
X!Tandem search:started runtandemsearch but it didn't complete
TPP: Once runtandemsearch completed, zztandempostproccessing.log file was full of warnings--WARNING: Mixture model quality test failed for charge (2+). WARNING: Mixture model quality test failed for charge (3+).

mzML

Conversion: File appears to have been converted
X!Tandem search: Tandem search completed
TPP: TPP analysis had warnings--WARNING: Mixture model quality test failed for charge (2+).

Personal tools