TPP TWA:Tutorial
From SPCTools
Contents |
Introduction
The TPP Web-launcher for Amazon (TWA) is a cloud computing web-based application for launching your own instance of TPP in the cloud. Based on Amazon Web Services, it simplifies the process of starting an Amazon Elastic Compute Cloud (EC2) instance with TPP already installed and ready to use.
About Tutorial
This tutorial is written for anyone interested in exploring using the Trans-Proteomics Pipeline on Amazon Web Services. It will walk the user through the steps of using TPP's Petunia web interface to setup proper Amazon credentials and submit multiple searches on AWS. Readers should already familiar with the TPP's web interface and usage.
Requirements
All that is required to execute this tutorial is a Amazon Web Services account, an Internet connection, and the current version of the supported web browsers, Firefox or Internet Explorer. Older browser versions or vendors may not support all functionality.
Tutorial
Readers should be aware that executing this tutorial will incur some AWS charges. The exact amount of these charges will vary based on a number of factors but should be on the order of $1-$4 USD.
Step 1: Getting Started
Launch the TWA application by navigating with your web browser to http://tools.proteomecenter.org/twa. The TWA web-based application will appear as a toolbar across the top of the default page with details about the TWA application.
Step 2: Getting Your Amazon credentials
In order for the TPP to have access to Amazon Web Services you must provide your AWS credentials confirm that you are who you say you are and that you do have permissions to do what you are trying to do. These credentials are known as Amazon's access and secret key. These keys are used to make secure REST or Query protocol requests to any AWS service API.
You can create new keys using the Amazon Security Credentials page. Your access keys are displayed under the Access Keys section in the Credentials Section of the page. Secret keys are now no longer displayed. If you've previously created a access/secret key pair and have forgotten what the secret key is you will need to generate a new key pair. For more information about setting up your AWS credentials please see Where's my secret access key?
Step 3: Signing into TWA
Step 4: Launch a EC2 instance
Step 5: Search data with X!Tandem
Step 7: Cleanup
After you are satisfied with your results its important to cleanup your files on Amazon to avoid any additional charges as the original input data and results remain in Amazon S3 until you perform this last step. Using Petunia navigate to Account > Amazon Cloud and click on the button "Shutdown all instances and delete all data". This will ensure that any remaining instances are terminated and all data stored in Amazon S3 is removed. Note at any time you can click on this button to stop Amazon processing and remove your data.
Next Steps
For further information on using Amazon cloud services see the other tutorials and documentation on this website or post your questions to the TPP mailing list at spctools-discuss discussion group.
