Software:Prequips:Inclusion List Builder
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| === Sample Data === | === Sample Data === | ||
| - | Download the ZIP archives below for a sample data set consisting of | + | Download the ZIP archives below for a sample data set to experiment with the Inclusion List Builder. |
| - | + | ||
| - | # high resolution mapping runs (3 mzXML files in profile mode) | + | |
| - | # peptide identifications for mapping runs (1 BioWorks tabular file) | + | |
| - | # inclusion list runs (5 mzXML files in profile mode) | + | |
| - | # peptide identifications for inclusion list runs (1 BioWorks tabular file) | + | |
| + | # High resolution mapping runs (3 mzXML files in profile mode, 151 MB). | ||
| + | # Inclusion list runs (5 mzXML files in profile mode, 107 MB). | ||
| + | # Peptide identifications for mapping and inclusion list runs (2 BioWorks tabular files, 2.1 MB). | ||
| == Prerequisites == | == Prerequisites == | ||
Revision as of 10:33, 24 September 2007
The Prequips Inclusion List Builder extension is a plug-in for Prequips. The Inclusion List Builder facilitates a directed LC-MS/MS that decouples peak detection and sequencing of selected precursor ions. The method has been described in detail in Schmidt A, Gehlenborg N, Bodenmiller B, Mueller L, Domon B and Aebersold R, "An integrated, directed mass spectrometric approach for in depth characterization of complex peptide mixtures." (submitted).
The Inclusion List Builders supports creation of inclusion lists and subsequent mapping of identified peptides to features.
Please contact Nils Gehlenborg if you experience problems with the Inclusion List Builder or Prequips.
Contents |
Concept
(to come)
Download
Software
We currently provide the Inclusion List Builder only bundled with Prequips.
- Download for Windows: Prequips 0.96 + Inclusion List Builder
- Download for Mac OS X: Prequips 0.96 + Inclusion List Builder
Sample Data
Download the ZIP archives below for a sample data set to experiment with the Inclusion List Builder.
- High resolution mapping runs (3 mzXML files in profile mode, 151 MB).
- Inclusion list runs (5 mzXML files in profile mode, 107 MB).
- Peptide identifications for mapping and inclusion list runs (2 BioWorks tabular files, 2.1 MB).
Prerequisites
- Make sure you have installed and configured a Java Runtime Environment (JRE) version 1.5 or later. Most machines should have this installed by default. Should your machine not have a recent JRE installed you may download a more up-to-date version from http://java.sun.com.
- Prequips requires large amounts of memory. We recommend at least 1024 MB.
Installation
- Extract the downloaded Prequips ZIP archive into a directory of your choice. A new subdirectory named "prequips_xxx" will be created ("xxx" will be replaced by the version and the OS identifier, e.g. "macosx" or "win32").
- Execute the Prequips application (prequips.app, prequips.exe, ...). A splash screen should appear within a few seconds.
Tutorial Screencasts
These tutorials require the free Apple Quicktime Player: http://www.apple.com/quicktime.
