Software:PeptideSieve

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-New native C++ version released 2/2008 (''link temporarily disabled until bugs fixed'')+New native C++ version (.51) released 5/2008
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 +[http://tools.proteomecenter.org/software/peptideSieve_080527.tar.gz]
 + 
 +Package contains linux, os x and windows binaries.
 + 
 +A windows UI version is available from our collaborator Chee-Hong! [http://web.bii.a-star.edu.sg/~wongch/peptideSieve] however it is running a slightly outdated version of sieve (.41) as of 5/27/08.
Build instructions are available [http://tools.proteomecenter.org/wiki/index.php?title=Software:PeptideSieve:Build here]. Build instructions are available [http://tools.proteomecenter.org/wiki/index.php?title=Software:PeptideSieve:Build here].

Revision as of 01:42, 28 May 2008

Peptide Sieve Package & Server

Important Note: PeptideSieve has been returned to public distribution now that it has been validated. Thank you for your patience.


Contents

Description

It has been noted [1] that only a handful of a protein’s possible tryptic peptides are consistently observed in proteomics experiments. We denote these consistently observed peptides to be proteotypic peptides. Such peptides have a variety of potential applications in proteomic research including improving protein identification scoring functions of database search software, providing a panel of reagents for protein quantification as well as the annotation of genomes for coding sequences of e.g. the hundreds of sequenced bacterial genomes some of which are important model organisms in systems biology and a guide for peptide selection in targeted proteomics experiments. Here we present PeptideSieve, an alpha version of a computational tool to predict a peptide’s proteotypic propensity based on its physico-chemical properties. The resulting predictors have the ability to accurately identify proteotypic peptides from any protein sequence and offer starting points for generating a physical model describing the factors that govern elements of proteomic workflows such as digestion, chromatography, ionization and fragmentation.

The software consists of a single C++ program. The input is either a FASTA file of protein sequences or a TXT file of peptide sequences. The program then returns which of a protein's peptides are most likely to be proteotypic for each of four common experimental designs.

Method outline

The program first performs an in silico digest of the protein and then converts each of the peptides into chemical property strings. The C++ program then computes a likelihood function, which scores the likelihood each peptide is proteotypic. It is important to note that the predictors are specific for particular experimental designs.

Reference

[1] Nat Biotechnol. 2007 Jan;25(1):125-31. Computational prediction of proteotypic peptides for quantitative proteomics. Mallick P, Schirle M, Chen SS, Flory MR, Lee H, Martin D, Ranish J, Raught B, Schmitt R, Werner T, Kuster B, Aebersold R.

Getting the software

New native C++ version (.51) released 5/2008

[1]

Package contains linux, os x and windows binaries.

A windows UI version is available from our collaborator Chee-Hong! [2] however it is running a slightly outdated version of sieve (.41) as of 5/27/08.

Build instructions are available here.

Predictions and Supplementary Materials

AEBERSOLD_TABLE_S1_Observed_Proteins

AEBERSOLD_TABLE_S2_Proteotypic_Peptides

AEBERSOLD_TABLE_S7_PAGE_ESI

AEBERSOLD_TABLE_S8_CZ_MALDI

AEBERSOLD_TABLE_S9_MUDPIT

AEBERSOLD_TABLE_S10_MUDPIT

AEBERSOLD_TABLE_S11_YEAST_PREDICTIONS

AEBERSOLD_TABLE_S12_HUMAN_PREDICTIONS

AEBERSOLD_TABLE_S13_EXTENDED_YEAST_PREDICTIONS

AEBERSOLD_TABLE_S14_EXTENDED_HUMAN_PREDICTIONS

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